mouse progranulin (R&D Systems)
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mouse progranulin
Mouse Progranulin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+pgrn/Mouse+Progranulin%2FPGRN+Antibody/10__1016_slash_j__isci__2026__115720-121-110-112
Average 93 stars, based on 94 article reviews
Mouse Progranulin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+pgrn/Mouse+Progranulin%2FPGRN+Antibody/10__1016_slash_j__isci__2026__115720-121-110-112
Average 93 stars, based on 94 article reviews
mouse progranulin - by Bioz Stars,
2026-10
93/100 stars
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Enzyme-linked Immunosorbent Assay:Article Title: Progranulin protects against endotoxin-induced acute kidney injury by downregulating renal cell death and inflammatory responses in mice. Article Snippet: a Department of Pathogenic Biology, Shandong University School of Medicine, Jinan, Shandong, PR China b Department of Pharmacology, Shandong University School of Medicine, Jinan, Shandong, PR China c Department of Biochemistry and Molecular Biology, Shandong University School of Medicine, Jinan, Shandong, PR China d Department of Hematology, Qilu Hospital of Shandong University, Jinan, Shandong, PR China e Department of Nephrology, Qilu Hospital of Shandong University, Jinan, Shandong, PR China Article Title: Progranulin drives acetaminophen-induced acute liver injury by limiting hepatic recruitment of eosinophils via suppressing interleukin-33 expression Article Snippet: The sections and cells were permeabilized with 0.5% Triton X-100 and then blocked with 30% goat serum (C0265, Beyotime Biotechnology, Shanghai, China) for 30 min at room temperature, and subsequently incubated with primary antibodies overnight at 4 ◦ C. Afterwards, they were incubated with a fluorescent secondary antibody and analyzed using Nikon AXR NSPARC laser confocal microscope (Nikon, Tokyo, Japan). .. Enzyme-linked immunosorbent assay (ELISA) kits for the measurement of human PGRN (DY2420), Incubation:Article Title: Reduced progranulin increases tau and α-synuclein inclusions and alters mouse tauopathy phenotypes via glucocerebrosidase. Article Snippet: The membranes were incubated in blocking buffer (Rockland MB-070) for 1 h at room temperature and then incubated overnight at 4 °C in blocking bufferwith primary antibodies: GCase (SIGMA #G4171, 1:1000) and FLAG (SIGMA #F7425, 1:1000), tau (DAKO #A0024, 1:5000), HT7 (Invitrogen #MN1000, 1:1000), AT8 (Invitrogen #MN1020, 1:1000), PHF1 (gift fromDr. .. Peter Davies, Albert Einstein College ofMedicine, Bronx, NY, 1:1000), phospho-tau (Ser199, Ser202) (Invitrogen #44-768G, 1:1000), phospho-tau (Ser356) (Invitrogen #44-751 G, 1:1000), β-actin (Cell Signaling Technology #3700, 1:2000), LIMPII (Novus Biologicals #NB400-129, 1:1000), cathepsin B (R&D #AF965, 1:500), cathepsin D (R&D #AF1029, 1:500), Hsp60 (Cell signaling #4870, 1:1000), calreticulin (Novus Biologicals #681233, 1:1000), Rab5 (Cell signaling #3547, 1:1000), Sap C (Santa Cruz #sc347118, 1:200), Article Title: Reduced progranulin increases tau and α-synuclein inclusions and alters mouse tauopathy phenotypes via glucocerebrosidase Article Snippet: The protein samples were electrophoresed using precast 4–20% Tris-glycine gels (Bio-Rad) and transferred with an iBlot 2 Transfer Device onto nitrocellulose membranes (Invitrogen IB23001). .. The membranes were incubated in blocking buffer (Rockland MB-070) for 1 h at room temperature and then incubated overnight at 4 °C in blocking buffer with primary antibodies: GCase (SIGMA #G4171, 1:1000) and FLAG (SIGMA #F7425, 1:1000), tau (DAKO #A0024, 1:5000), HT7 (Invitrogen #MN1000, 1:1000), AT8 (Invitrogen #MN1020, 1:1000), PHF1 (gift from Dr. Peter Davies, Albert Einstein College of Medicine, Bronx, NY, 1:1000), phospho-tau (Ser199, Ser202) (Invitrogen #44-768 G, 1:1000), phospho-tau (Ser356) (Invitrogen #44-751 G, 1:1000), β-actin (Cell Signaling Technology #3700, 1:2000), LIMPII (Novus Biologicals #NB400-129, 1:1000), cathepsin B (R&D #AF965, 1:500), cathepsin D (R&D #AF1029, 1:500), Hsp60 (Cell signaling #4870, 1:1000), calreticulin (Novus Biologicals #681233, 1:1000), Rab5 (Cell signaling #3547, 1:1000), Sap C (Santa Cruz #sc-347118, 1:200), Recombinant:Article Title: Progranulin attenuates liver fibrosis by downregulating the inflammatory response. Article Snippet: .. Recombinant human PGRN, Blocking Assay:Article Title: Reduced progranulin increases tau and α-synuclein inclusions and alters mouse tauopathy phenotypes via glucocerebrosidase Article Snippet: The protein samples were electrophoresed using precast 4–20% Tris-glycine gels (Bio-Rad) and transferred with an iBlot 2 Transfer Device onto nitrocellulose membranes (Invitrogen IB23001). .. The membranes were incubated in blocking buffer (Rockland MB-070) for 1 h at room temperature and then incubated overnight at 4 °C in blocking buffer with primary antibodies: GCase (SIGMA #G4171, 1:1000) and FLAG (SIGMA #F7425, 1:1000), tau (DAKO #A0024, 1:5000), HT7 (Invitrogen #MN1000, 1:1000), AT8 (Invitrogen #MN1020, 1:1000), PHF1 (gift from Dr. Peter Davies, Albert Einstein College of Medicine, Bronx, NY, 1:1000), phospho-tau (Ser199, Ser202) (Invitrogen #44-768 G, 1:1000), phospho-tau (Ser356) (Invitrogen #44-751 G, 1:1000), β-actin (Cell Signaling Technology #3700, 1:2000), LIMPII (Novus Biologicals #NB400-129, 1:1000), cathepsin B (R&D #AF965, 1:500), cathepsin D (R&D #AF1029, 1:500), Hsp60 (Cell signaling #4870, 1:1000), calreticulin (Novus Biologicals #681233, 1:1000), Rab5 (Cell signaling #3547, 1:1000), Sap C (Santa Cruz #sc-347118, 1:200), |
![Increased protein levels <t>of</t> <t>PSAP</t> and <t>PGRN</t> within the SFO of SAP-D −/− mice a – b) , Coronal brain section at 0.7–0.8 mm posterior to bregma containing the SFO. a: DAPI staining. a-2: Enlarged view of the white square in a-1. SFO: subfornical organ, 3V: third ventricle. b; Double immunofluorescent staining of PSAP (red) and PGRN (green) in 10-month-old female WT and SAP-D −/− mice. DAPI (blue) staining showed the nuclei ( a and b ). Scale bar, 500 μm ( a and b ). White arrowheads indicate the SFO regions ( b ). c) Cerebral region from 3-, 6-, and 10-month-old male and female mice containing the SFO (0.7–0.8 mm posterior to ∗bregma) used for protein extraction and Western blot ( d – h ) using anti- PSAP, PGRN, and GAPDH antibodies. Quantification normalized to GAPDH expression and represented as the mean ± SD of three mice for each group. and indicate the individual values in each group ( e , f , h ). d – f) PSAP and PGRN protein levels in the SFO were remarkably increased. Their quantification by densitometric analysis is represented in e for male and f for female, respectively. e ) For PSAP/GAPDH, two-way ANOVA revealed a significant main effects of genotype (F(1,24) = 547.7, p < 0.0001, ηp 2 = 0.48, 95 % CI [−74.51, −62.43]), with no effect of age ( p = 0.46) or genotype × age interaction ( p = 0.47). Post-hoc Tukey's tests showed that SAP-D −/− differed from WT at 3 M ( p < 0.0001, Cohen's d = 9.99, 95 % CI [−86.30, −54.96]), 6 M ( p < 0.0001, Cohen's d = 7.24, 95 % CI [−79.04, −47.70]), and 10 M ( p < 0.0001, Cohen's d = 8.77, 95 % CI [−87.09, −55.75]). For PGRN/GAPDH, two-way ANOVA revealed a significant main effect of genotype (F(1,24) = 354.2, p < 0.0001, ηp 2 = 0.48, 95 % CI [−46.39, −37.22]), with no effect of age ( p = 0.73) or genotype × age interaction ( p = 0.76). Post-hoc Tukey's tests showed that SAP-D −/− differed from WT at 3 M ( p < 0.0001, Cohen's d = 7.87, 95 % CI [−52.71, −28.91]), 6 M ( p < 0.0001, Cohen's d = 5.35, 95 % CI [−52.82, −29.02]), and 10 M ( p < 0.0001, Cohen's d = 8.77, 95 % CI [−52.32, −28.52]). f ) For PSAP/GAPDH, two-way ANOVA revealed a significant main effect of genotype (F(1,24) = 611.1, p < 0.0001, ηp 2 = 0.48, 95 % CI [−74.42, −62.95]), with no effect of age ( p = 0.07) or genotype × age interaction (p = 0.07). Post-hoc Tukey's tests showed that SAP-D −/− differed from WT at 3 M ( p < 0.0001, Cohen's d = 9.99, 95 % CI [−86.30, −54.96]), 6 M ( p < 0.0001, Cohen's d = 7.24, 95 % CI [−79.04, −47.70]), and 10 M ( p < 0.0001, Cohen's d = 8.77, 95 % CI [−87.09, −55.75]). For PGRN/GAPDH, two-way ANOVA revealed a significant main effect of genotype (F(1,24) = 273.5, p < 0.0001, ηp 2 = 0.47, 95 % CI [−63.96, −49.76]), with no effect of age ( p = 0.71) or genotype × age interaction (p = 0.70). Post-hoc Tukey's tests showed that SAP-D −/− differed from WT at 3 M ( p < 0.0001, Cohen's d = 7.31, 95 % CI [−73.43, −36.60]), 6 M ( p < 0.0001, Cohen's d = 5.71, 95 % CI [−73.04, −36.22]), and 10 M ( p < 0.0001, Cohen's d = 5.35, 95 % CI [−73.08, −36.26]). g – h ) Comparison of PSAP and PGRN protein expression in the SFO, whole cerebrum, and cerebellum. The quantitative analysis is shown in h . h ) For PSAP/GAPDH, one-way ANOVA revealed a significant effect in SAP-D −/− mice (F(2,6) = 30.06, p = 0.0007, η 2 = 0.90), but not in WT mice ( p = 0.3461). Tukey's post hoc tests showed significant differences for SFO versus cerebellum ( p = 0.0010, Cohen's d = 5.26, 95 % CI [46.80, 119.1]) as well as and cerebrum versus cerebellum ( p = 0.0018, Cohen's d = 9.34, 95 % CI [38.39, 110.6]). There was no significant difference for SFO versus cerebrum ( p = 0.76, Cohen's d = 0.47, 95 % CI [−27.71, 44.54]). For PGRN/GAPDH, one-way ANOVA revealed a significant effect in SAP-D −/− mice (F(2,6) = 54.42, p = 0.0001, η 2 = 0.94), but not in WT mice ( p = 0.6327). Tukey's post hoc tests showed significant differences for SFO versus cerebrum ( p = 0.0005, Cohen's d = 5.43, 95 % CI [26.19, 57.84]) as well as and SFO versus cerebellum ( p = 0.0005, Cohen's d = 5.43, 95 % CI [26.19, 57.84]), but not for cerebrum versus cerebellum ( p = 0.3274, Cohen's d = 6.06, 95 % CI [−7.72, 23.92]). ns: no significant difference. ∗∗∗∗ p < 0.0001. ∗∗∗ p < 0.001. ∗∗ p < 0.01.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0004/pmc12720004/pmc12720004__gr1.jpg)
